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sci.abbe-diffraction-limit Calculator
Calculates the Abbe diffraction resolution limit for an optical microscope: d = λ/(2 NA), where NA is numerical aperture. Oil-immersion objective with NA = 1.4 at 550 nm: d = 196 nm — super-resolution techniques (STED, PALM, STORM) break this limit by exploiting fluorophore photophysics.
Inputs
Lambda Nm
Distance between successive wave crests. Related to frequency: λ = speed / frequency.
Na Obj
Reference formula or conversion factor shown for context.
N Medium
Reference formula or conversion factor shown for context.