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sci.ELISA-OD-concentration Calculator
Calculates analyte concentration from ELISA optical density using a standard curve and accounts for dilution factor. 4-parameter logistic (4PL) curve fitting is standard for ELISA — the dynamic range spans the linear region between upper and lower asymptotes.
Inputs
Od Sample
Reference formula or conversion factor shown for context.
Od Blank
Reference formula or conversion factor shown for context.
Od Std1
Reference formula or conversion factor shown for context.
C Std1 Ng Ml
Amount of solute per unit volume. Higher concentration = more solute dissolved.
Od Std2
Reference formula or conversion factor shown for context.
C Std2 Ng Ml
Amount of solute per unit volume. Higher concentration = more solute dissolved.
Results
sample concentration (ng/mL)
The proportional relationship between two quantities.
net sample OD
Sample size or count used in the calculation.
standard curve slope
The gradient -- rise divided by run. In finance, rate of change per unit time.
linear regression: C = slope×OD + intercept
The line of best fit through the data points. Used to predict output from input.
dynamic range
The difference between the maximum and minimum values.
QC check
A pass/fail verification against the applicable code, standard, or threshold.