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sci.enzyme-turnover-kcat Calculator
Calculates enzyme turnover number kcat = Vmax/[E_total] and catalytic efficiency kcat/Km from kinetic data. The diffusion limit for kcat/Km is ~10⁸–10⁹ M⁻¹s⁻¹ — catalytically perfect enzymes like carbonic anhydrase (kcat/Km = 4×10⁷) operate near this limit.
Inputs
Vmax Nmol Min Mg
Reference formula or conversion factor shown for context.
Molecular Weight Kda
Body or object weight. For health calculators, usually in kg or lbs.
Km Mm
Reference formula or conversion factor shown for context.
Results
turnover number kcat (s⁻¹)
The value at the specified point or condition.
catalytic efficiency kcat/Km (M⁻¹s⁻¹)
Useful output divided by total input, as a percentage. True 100% efficiency is impossible — losses appear as heat. LEDs: 30–50%. Electric motors: 85–97%. Switching supplies: 85–95%.