// multi-utility computation suite · offline · instant · precise
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│ computation suite │
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sci.gc-content-dna Calculator
Calculates GC content of a DNA sequence and estimates melting temperature: Tm = 81.5 + 16.6 log[Na⁺] + 0.41(%GC) − 675/n. GC pairs form 3 hydrogen bonds vs 2 for AT — higher GC content raises Tm; thermophilic bacteria have >70% GC in heat-stable genes.
Inputs
Adenine Count
Reference formula or conversion factor shown for context.
Thymine Count
Reference formula or conversion factor shown for context.
Guanine Count
Reference formula or conversion factor shown for context.
Cytosine Count
Reference formula or conversion factor shown for context.
Results
GC content (%)
Sample size or count used in the calculation.
AT content (%)
The value at the specified point or condition.
sequence length (bp)
The computed linear length.
estimated Tm (°C) at 50mM Na⁺
The value at the specified point or condition.
A:T ratio
The proportional relationship between two quantities.
Wallace rule Tm
Reference formula or conversion factor shown for context.