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sci.hplc-resolution Calculator
Calculates HPLC column resolution Rs = (t₂ − t₁)/(0.5(w₁ + w₂)) and the plate count needed for baseline separation. Rs > 1.5 is baseline resolution — peak resolution scales as √N, so doubling column length improves Rs by only 41%.
Inputs
Retention Time1 Min
Duration of the process. Make sure units match the rate inputs (seconds, minutes, or hours).
Retention Time2 Min
Duration of the process. Make sure units match the rate inputs (seconds, minutes, or hours).
Peak Width1 Min
Horizontal extent perpendicular to the length.
Peak Width2 Min
Horizontal extent perpendicular to the length.
Results
resolution Rs
Sample size or count used in the calculation.
plate count N₁
Total number of items or occurrences.
plate count N₂
Total number of items or occurrences.
separation quality
A qualitative assessment of how the result compares to the desired standard or benchmark.
Rs = 2(t₂-t₁)/(w₁+w₂)
Reference formula or conversion factor shown for context.
Rs ≥ 1.5
Reference formula or conversion factor shown for context.