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sci.numerical-aperture-lens Calculator
Calculates numerical aperture NA = n sin θ of an objective lens and the resulting lateral resolution limit. NA = 1.4 oil-immersion objective achieves 0.2 µm lateral resolution at 550 nm — the resolution limit of light microscopy before super-resolution techniques.
Inputs
Medium Refractive Index
Reference formula or conversion factor shown for context.
Half Angle Deg
Reference formula or conversion factor shown for context.
Results
numerical aperture NA
Sample size or count used in the calculation.
Rayleigh resolution (λ=550nm, µm)
Sample size or count used in the calculation.
depth of field (µm)
The computed depth.
NA = n·sin(θ)
Sample size or count used in the calculation.
microscope objectives
Reference formula or conversion factor shown for context.