// multi-utility computation suite · offline · instant · precise
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sci.pcr-amplification Calculator
Calculates the number of PCR cycles needed to amplify a target from template count to detection threshold: copies = template × 2^n. 30 PCR cycles amplify 10 copies to ~10 billion — efficiency below 100% reduces effective amplification.
Inputs
Initial Copies
Reference formula or conversion factor shown for context.
Cycles
Reference formula or conversion factor shown for context.
Efficiency Pct
Useful output divided by total input, as a percentage. 100% is impossible due to losses (heat, friction, resistance).
Results
DNA copies after PCR
Sample size or count used in the calculation.
fold amplification
The value at the specified point or condition.
cycles
Reference formula or conversion factor shown for context.
efficiency
Useful output divided by total input, as a percentage. True 100% efficiency is impossible — losses appear as heat. LEDs: 30–50%. Electric motors: 85–97%. Switching supplies: 85–95%.
theoretical (100% eff)
Reference formula or conversion factor shown for context.