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sci.PCR-efficiency-calculation Calculator
Calculates PCR amplification efficiency E from the standard curve slope: E = 10^(−1/slope) − 1, with E = 1.0 (100%) being ideal doubling per cycle. PCR efficiency below 90% suggests inhibitors or poor primer design — efficiency above 110% indicates primer dimers or multiple amplicons.
Inputs
Ct Efficiency 1
Reference formula or conversion factor shown for context.
Ct Efficiency 2
Reference formula or conversion factor shown for context.
Ct Sample
Reference formula or conversion factor shown for context.
Ct Reference
Reference formula or conversion factor shown for context.
Results
PCR efficiency E
Useful output divided by total input, as a percentage. True 100% efficiency is impossible — losses appear as heat. LEDs: 30–50%. Electric motors: 85–97%. Switching supplies: 85–95%.
slope per 10× dilution
The gradient -- rise divided by run. In finance, rate of change per unit time.
ΔΔCt (sample - reference)
Sample size or count used in the calculation.
relative expression ratio
The proportional relationship between two quantities.
corrected ratio (using E)
The proportional relationship between two quantities.
E = 10^(-1/slope) - 1
The gradient -- rise divided by run. In finance, rate of change per unit time.