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sci.protein-molecular-weight Calculator
Calculates protein molecular weight from amino acid sequence composition using average residue mass ≈ 110 Da, and from SDS-PAGE migration. Myoglobin: 17 kDa; haemoglobin subunit: 16 kDa; IgG antibody: 150 kDa — SEC-MALS and mass spectrometry give more accurate masses than SDS-PAGE.
Inputs
Amino Acids
Count of items or occurrences.
Avg Residue Mw
Reference formula or conversion factor shown for context.
Disulfide Bonds
Count of items or occurrences.
Results
molecular weight (kDa)
The computed weight (gravitational force) or mass.
molecular weight (Da)
The computed weight (gravitational force) or mass.
amino acid residues
Sample size or count used in the calculation.
average residue MW
The arithmetic mean -- sum divided by count.
+18 Da for water (termini)
The value at the specified point or condition.
SDS-PAGE
Reference formula or conversion factor shown for context.