// multi-utility computation suite · offline · instant · precise
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sci.spectroscopy-absorbance-beer Calculator
Calculates analyte concentration from UV-Vis absorbance using the Beer-Lambert law: A = ε c l. Molar extinction coefficient ε of protein at 280 nm ≈ 1 AU·L/(g·cm) — DNA concentration at 260 nm: 1 OD260 ≈ 50 µg/mL for double-stranded DNA.
Inputs
Concentration Mol L
Amount of solute per unit volume. Higher concentration = more solute dissolved.
Path Length Cm
Linear measurement. Ensure consistent units: 1 m = 1,000 mm = 3.281 ft.
Molar Absorptivity L Mol Cm
Reference formula or conversion factor shown for context.
Results
absorbance A
Sample size or count used in the calculation.
transmittance T
Sample size or count used in the calculation.
%T
Reference formula or conversion factor shown for context.
Beer-Lambert: A = ε·b·C
Reference formula or conversion factor shown for context.